Assessment of Gold Nanomaterial Uptake in HepG2 Cells by Single Particle ICP-MS and ICP-MS |
SP-ICP-MS 및 ICP-MS를 이용한 HepG2 세포 내금 나노물질 축적 평가 |
김은정, 성화경, 유선경, 조은혜, 김현미, 김필제, 유승도 |
국립환경과학원 |
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Abstract |
The cellular uptake of gold nanomaterials (AuNPs) was investigated in human liver HepG2 cells exposed to 0.5 or 1 µg/mL of AuNPs for 24 hours. After the exposure period, the total gold concentration in the medium and cells was observed to have increased significantly in a dose-dependent manner; gold nanomaterials were mainly present in the medium, while some were present in the cells. SP-ICP-MS showed that the AuNPs concentration increased in the medium and cells, but the results were lower in comparison to the total gold concentration measured by ICP-MS. This difference was presumably due to the fact that AuNPs were ionized and aggregated, and only a part of them retained a shape that is detectable by SP-ICP-MS. The median size of AuNPs remained similar after 24 hours to that at initial exposure. In conclusion, this study confirmed the applicability of SP-ICP-MS for the analysis of the concentration and size of AuNPs and the distribution pattern of AuNPs in HepG2 cells |
Key Words:
Gold nanomaterials, Uptake, HepG2 cells, SP-ICP-MS, ICP-MS |
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