| In Vivo Micronucleus Test of Methylcyclopentane and 1,4-Dichlorobutane |
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| Abstract |
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We investigated the genotoxicity of two chemicals, methylcyclopentane and 1,4-dichlorobutane with in vivo micronucleus test. Although these two chemicals have already been tested many times, a micronucleus test has not been conducted and the usage of these chemicals has been recently increased. 7 week male ICR mice were tested at dosages of 500, 1,000, and 2,000 mg/kg for methylcyclopentane and 500, 1,000, and 2,000 mg/kg for 1,4-dichlorobutane, respectively. After 24 hours of oral administration with the two chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes (PCE), all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these two chemicals. It was concluded that the two chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the two chemicals have no mutagenic potential under each study condition. |
| Key Words:
Bone marrow, Methylcyclopentane, Micronucleus test, 1,4-Dichlorobutane |
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