Simultaneous Analysis of Triazines in Human Urine

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Research article

Journal of the Korean Society for Environmental Analysis 2010;13(3):149-160.

뇨중 Triazine류의 동시분석

황선혜, 김승기, 최종호

한국과학기술연구원

Abstract

Triazines are the widely used herbicides and they can be introduced into rivers or lakes. When a human body is exposed to triazines, nausea, vomiting, dyspnea and nephritis can be induced. Therefore, the ability to detect triazine by qualitative analysis is required. In this study, an analytical method for 10 triazines including simazine, prometon, atrazine, propazine, terbuthylazine, simetryn, ametryn, prometryn, terbutryn and cyanazine was investigated. Liquid-liquid extraction of the triazines from human urine and analysis of the compounds using gas chromatograph-mass spectrometry (GC-MS) and liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS) were conducted. In the case of urine samples, extraction recoveries of triazines were within the range of 71.99-111.91%. The calibration curves showed a good linearity (R2 of 0.998-0.999)and method detection limits of all the analytes were obtained in the range of 0.10-0.95 ng/mL by the signal to noise method (S/N>3). Instrument detection limits of the LC-MS/MS were within the range of 0.01-0.25 pg.

Key Words: Triazines, Liquid-liquid extraction, GC-MS, LC-MS/MS, Urine