J Korean Soc Environ Anal Search


Journal of the Korean Society for Environmental Analysis 2009;12(2):130-135.
Separation Characteristics of Polychlorinated Biphenyls (PCBs) on Two Naphthalenecarboxamide Bonded Silica Stationary Phases
In this study, we synthesized two new stationary phases based on naphthalenecarboxamide isomers and investigated the separation characteristics of 62 PCBs resulted from π-π interaction and steric repulsion. Two stationary phases(1- and 2-naphthalenecarboxamide) were simply prepared by coupling reaction of acids(1- and 2-naphthoic acid) and amionopropyl silica gel with EEDQ(2-Ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline) as coupling reagent. The fractionation of PCBs were performed using high performance liquid chromatography (HPLC) equipped with two new stationary phase columns and compared with commercially available 2-(1-pyrenyl)ethyldimethylsilyated (PYE) and octadecyl (C18) stationary phase column. As a consequence, we could identify PCBs which had been eluted in the HPLC fractions using gas chromatography/ mass spectrometer (GC/MS) and confirm that 2-naphthalenecarboxamide stationary phase was more efficient to separated PCBs than 1-naphthalenecarboxamide stationary phase. The retention capacity was increased according to the planarity of PCBs and especially 3,3’,4,4’,5-pentaCB (IUPAC No. 126) was eluted in the last fraction. In the case of stationary phase, the retention of PCBs was as follows; PYE > 2-naphthalenecarboxamide > 1-naphthalenecarboxamide > C18. This result means that the difference of π-electrons conjugation and isomeric molecular structure of stationary phases may determine the separation characteristics of PCBs in HPLC fractionation.
Key Words: PCBs, HPLC, Stationary Phases, Naphthalenecarboxamide


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