Separation Characteristics of Polychlorinated Biphenyls (PCBs) on Two Naphthalenecarboxamide Bonded Silica Stationary Phases

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Research article

Journal of the Korean Society for Environmental Analysis 2009;12(2):130-135.

Abstract

In this study, we synthesized two new stationary phases based on naphthalenecarboxamide isomers and investigated the separation characteristics of 62 PCBs resulted from π-π interaction and steric repulsion. Two stationary phases(1- and 2-naphthalenecarboxamide) were simply prepared by coupling reaction of acids(1- and 2-naphthoic acid) and amionopropyl silica gel with EEDQ(2-Ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline) as coupling reagent. The fractionation of PCBs were performed using high performance liquid chromatography (HPLC) equipped with two new stationary phase columns and compared with commercially available 2-(1-pyrenyl)ethyldimethylsilyated (PYE) and octadecyl (C18) stationary phase column. As a consequence, we could identify PCBs which had been eluted in the HPLC fractions using gas chromatography/ mass spectrometer (GC/MS) and confirm that 2-naphthalenecarboxamide stationary phase was more efficient to separated PCBs than 1-naphthalenecarboxamide stationary phase. The retention capacity was increased according to the planarity of PCBs and especially 3,3’,4,4’,5-pentaCB (IUPAC No. 126) was eluted in the last fraction. In the case of stationary phase, the retention of PCBs was as follows; PYE > 2-naphthalenecarboxamide > 1-naphthalenecarboxamide > C18. This result means that the difference of π-electrons conjugation and isomeric molecular structure of stationary phases may determine the separation characteristics of PCBs in HPLC fractionation.

Key Words: PCBs, HPLC, Stationary Phases, Naphthalenecarboxamide