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Journal of the Korean Society for Environmental Analysis 2008;11(1):29-38.
Acidic Mine drainage Analysis based on Bioassay
생태독성법을 이용한 산성배수 평가
전태성1, 연익준1, 박종호2, 최현일3
1충주대학교
2충청북도내수면연구소
3포스코건설㈜
Abstract
Acidic shale mine drainage (ASMD) contains high content of mineral ions such as Al, Fe, and Mg, while its turbidity is low (0.4-0.6 NTU). Yellowish brown precipitation of iron hydroxide in valley deteriorates the natural views. ASMD results in changes in aquatic environments and the reduction of diversity and population size of aquatic ecosystem. Also, white colored precipitation at the point of ASMD injection to main stream might be originated by aluminium ion in ASMD. This causes physicochemical and social problem to users of this stream as drinking water source and irrigation water. Thus, ASMD in valley has to be neutralized and treated for the removal of mineral ions, before discharging to main water stream. The purpose of this research focused on evaluating various bioassay. Carassius auratus and Pungtungia herzi were exposed to mercury (HgCl₂), lead (PbCl₂) and copper (CuSO₄) during larval stage, more sensitive than other life stages to determine acute toxicity. The experiments were repeated three times and the LC50 (24, 48, 72 and 96 hour) was determined for two species. Data obtained from the acute toxicity tests were evaluated using the probit analysis statistical method. C. auratus was found to be more susceptible than P. herzi to lead, while P. herzi was found to be more sensitive than C. auratus for mercury and copper. The sensitivity of three metals tested is likely ranked in the following order from highest to lowest toxicity on larval stage of these fish: mercury > copper > lead. It is suggested that acute toxic test at the larval stage of C. auratus and P. herzi which are indigenous species in Asia area is an important part of the ecotoxicological assessment of some heavy metals.
Key Words: ASMD, Carassius auratus, Pungtungia herzi, mercury, lead, copper, acute toxicity tests
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