Bioelectrochemical Fixation of CO₂using Autotrophic and Mixotrophic CO₂-Fixing Bacteria |
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Abstract |
For the isolation of CO₂-fixing bacteria using electrochemical reducing power (ERP), a plate-type electrochemical bioreactor (PEB) was employed. Soluble neutral red (NR) was used as an electron mediator for carbonate-basal agar medium (CBAM) prepared in the PEB. For test of bacterial CO₂ fixation using the ERP,a single-compartmented electrochemical bioreactor (SCEB) was employed. NR immobilized in the graphite felt cathode (NR-cathode) was used as an electron mediator for carbonate-basal broth medium (CBBM) prepared in the SCEB. Two bacterial genera capable of electrochemically fixing CO₂were isolated using the PEB and cultivated using the SCEB. The isolated bacterial species were 99% identified with Achromobacter sp. and Alcaligenes sp. Approximate 150 mL of CO₂per day was consumed by 300 mL of the mixed culture of Achromobacter sp. and Alcaligenes sp. grown electrochemically in the SCEB. |
Key Words:
CO₂-fixing bacteria, neutral red, electrochemical reducing power, Alcaligenes sp., Achromobacter sp. |
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